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Khanh Huy Bui Anatomy and Cell Biology

Khanh Huy Bui investigates the molecular architecture of the cilium, hair-like structures attached to the surface of almost all cells in the human body, responsible for sensory reception and mucociliary clearance. Defects in cilia cause numerous diseases and developmental disorders including chronic respiratory infection, obesity, blindness, and intellectual disability. The challenge in understanding the function of the cilium is that it is one of the most complicated but orderly built organelles in the cell, composed of more than 500 unique protein molecules. Most of these unique proteins’ functions and locations are unknown. Research in the Bui lab attempts to solve this molecular puzzle. Using a combination of cryo-electron microscopy, proteomics, and molecular genetics, we aim to create the molecular map of all the ciliary proteins at near atomic resolution in order to infer how its architecture allows its intricate motility and complex regulation. In addition, we use correlative light and electron microscopy to look at the assembly process of the cilium, a dynamic process, which is not amenable to traditional structural techniques.

Awards

Tier 2 Canada Research Chair in cryo-electron tomography

AusAID undergraduate scholarship

Relevant Publications

M. Ichikawa, D. Liu, P.L. Kastritis, K. Basu, T.C. Hsu, S. Yang, K.H. Bui, "Subnanometer- resolution structure of the doublet microtubule reveals new classes of microtubule- associated proteins," Nature Comms, 8: 15035, 2017.

A. von Appen, J. Kosinski, L. Sparks, A. Ori, A.L. DiGuilio, B. Vollmer, M. Mackmull, N. Banterle, L. Parca, K. Buczak, S. Mosalaganti, W. Hagen, W. Andres-Pons, E.A. Lemke, P. Bork, W. Antonin, J.S. Glavy, K.H. Bui**, M. Beck**, "In situ structural analysis of the human nuclear pore complex," Nature, 526: 140-143, 2015. (** Shared corresponding authors).

K.H. Bui, A. von Appen, A.L. DiGuilio, A. Ori, L. Sparks, M. Mackmull, T. Bock, W. Hagen, A. Andres-Pons, J.S. Glavy, M. Beck, "Integrated structural analysis of the human nuclear pore complex scaffold," Cell 155:1233-43, 2013.

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Appointment

CIFAR Azrieli Global Scholar Molecular Architecture of Life

Institution

McGill University

Education

Ph.D. Swiss Federal Institute of Technology, Zurich, Switzerland

MSc. Chalmers University of Technology, Sweden

BSc University of New South Wales, Australia

Country

Canada

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