Khanh Huy Bui Anatomy and Cell Biology
Khanh Huy Bui investigates the molecular architecture of the cilium, hair-like structures attached to the surface of almost all cells in the human body, responsible for sensory reception and mucociliary clearance. Defects in cilia cause numerous diseases and developmental disorders including chronic respiratory infection, obesity, blindness, and intellectual disability. The challenge in understanding the function of the cilium is that it is one of the most complicated but orderly built organelles in the cell, composed of more than 500 unique protein molecules. Most of these unique proteins’ functions and locations are unknown. Research in the Bui lab attempts to solve this molecular puzzle. Using a combination of cryo-electron microscopy, proteomics, and molecular genetics, we aim to create the molecular map of all the ciliary proteins at near atomic resolution in order to infer how its architecture allows its intricate motility and complex regulation. In addition, we use correlative light and electron microscopy to look at the assembly process of the cilium, a dynamic process, which is not amenable to traditional structural techniques.
Tier 2 Canada Research Chair in cryo-electron tomography
AusAID undergraduate scholarship
M. Ichikawa, D. Liu, P.L. Kastritis, K. Basu, T.C. Hsu, S. Yang, K.H. Bui, "Subnanometer- resolution structure of the doublet microtubule reveals new classes of microtubule- associated proteins," Nature Comms, 8: 15035, 2017.
A. von Appen, J. Kosinski, L. Sparks, A. Ori, A.L. DiGuilio, B. Vollmer, M. Mackmull, N. Banterle, L. Parca, K. Buczak, S. Mosalaganti, W. Hagen, W. Andres-Pons, E.A. Lemke, P. Bork, W. Antonin, J.S. Glavy, K.H. Bui**, M. Beck**, "In situ structural analysis of the human nuclear pore complex," Nature, 526: 140-143, 2015. (** Shared corresponding authors).
K.H. Bui, A. von Appen, A.L. DiGuilio, A. Ori, L. Sparks, M. Mackmull, T. Bock, W. Hagen, A. Andres-Pons, J.S. Glavy, M. Beck, "Integrated structural analysis of the human nuclear pore complex scaffold," Cell 155:1233-43, 2013.
CIFAR Azrieli Global Scholar Molecular Architecture of Life
Ph.D. Swiss Federal Institute of Technology, Zurich, Switzerland
MSc. Chalmers University of Technology, Sweden
BSc University of New South Wales, Australia
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